20mg Package
Recombinant Protein A

Formulation: Lyophilized Salt-free powder
Product#: A100L20
 
$53.00 USD each

Add to Cart

 

150mg Package
Recombinant Protein A

Formulation: Lyophilized Salt-free powder
Product#: A100L150
 
$156.00 USD each

Add to Cart

 

Bulk Size
Recombinant Protein A

Formulation: Lyophilized Salt-free powder
 
 
Available upon request

Bulk Quote

MPT Biotech
 
Frost & Sullivan 2012 Best Practices Award

Recombinant Protein A

Protein A is a cell wall protein derived from Staphylococcus aureus. This protein exhibits unique binding properties for the Fc region of IgG/antibodies from a variety of mammalian species as well as some IgM and IgA, and diverse recombinant antibody fragments and constructs retaining a binding region. It also couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. In addition, Protein A can be immobilized on a solid support, such as agarose or acrylic beads, to purify immunoglobulins/antibodies. It can also be used in immunoprecipitation assays.

Bulk Protein A is available for 3rd party manufacture of resins. Please contact us for further information.

Protein Description

Proprietary recombinant Protein A is produced using our proprietary MPTxpress solid-state E. coli expression system. This Protein A functions essentially the same as native Protein A in terms of antibody affinity, but has greatly increased pH tolerance, and conjugated resins can be recycled many more times under more extreme alkaline conditions. Recombinant Staphylococcal Protein A, produced using our proprietary E. coli strain and vectors, is a non-glycosylated polypeptide chain containing 290 amino acids, and has an approximate molecular mass of 35.1 kDa. It consists of 5 IgG binding domains E-D-A-B-C aligned in series along with slight N- and C-terminal modifications relative to the native protein. A specific purification process with strict quality control is used to isolate recombinant Protein A to >95% purity as a salt-free lyophilized powder. No animal-derived products are used in bioprocessing. Our proprietary upstream MPTxpress E. coli platform is liquid- and stress-free and devoid of bacterial contaminants normally found in native and some other recombinant Protein A products, including being free of Staphylococcus endotoxins and hemolysin. The product is consistent in quality and yield, with the MPTxpress manufacturing platform deficient in major proteolytic activities. Recombinant Protein A is purified by proprietary chromatographic methods to purity greater than 95%.

Source

Escherichia coli cultured using the MPTxpress platform

Storage

Store at 4°C if entire vial will be used within 5 weeks. Store, frozen at -20°C for months longer periods. After reconstitution in distilled water or saline, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Purity and Formulation

≥95% by SDS-Page and HPLC analyses. Lyophilized powder with no additives.

Usage

MicroProtein’s material is furnished for laboratory or bioprocessing purposes only. Inquire about Protein A for human or animal applications.

Purchase

20mg Package
Recombinant Protein A

Formulation: Lyophilized Salt-free powder
Product#: A100L20
 
$53.00 USD each

Add to Cart

 

150mg Package
Recombinant Protein A

Formulation: Lyophilized Salt-free powder
Product#: A100L150
 
$156.00 USD each

Add to Cart

 

Bulk Size
Recombinant Protein A

Formulation: Lyophilized Salt-free powder
 
 
Available upon request

Bulk Quote