Streptavidin

Streptavidin is a biotin-binding protein found in culture broth of the bacterium Streptomyces avidin. Streptavidin binds 4 moles of biotin per mole of protein with a remarkably high affinity. MicroProtein’s recombinant Streptavidin lacks carbohydrate side chains or other associated cofactors. Streptavidin has isoelectric point near to neutrality where most useful biological interaction occur. Accordingly, streptavidin exhibits lower levels of non-specific interaction than does avidin molecule when the streptavidin protein used in applications relying upon the formation of avidin/biotin complexes. Streptavidin is a 52 K Dalton, is a tetramer complex composed of 4 identical polypeptide chains. Recombinant Streptavidin protein contain no cysteine residues, sugar side chains or other cofactors. Streptavidin protein is stable over wide pH range and heat stable, heating up to 100°C for 20 minutes in 0.2% SDS to dissociate the subunits.

Product Code

MPT-SA-100 , Specific Activity 15 U/mg

Applications

MicroProtein’s high quality recombinant Streptavidin is a suitable tool for allowing universal test systems in molecular diagnostics, reagents, kits, and immunology. This recombinant Streptavidin is appropriate for coating solid phases such as microarrays, beads, and microplates etc.

Formulation

Lyophilized salt free.

Stability

Streptavidin stored dry at 4°C to -20°C
Heating up to 100°C

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